Veterinary Toxicology is a broad area of science, which deals with diagnosis and treatment of intoxications in pets, livestock, and wildlife species. The Toxicology Diagnostics Laboratory at the Agricultural Research Council - Onderstepoort Veterinary Research (ARC-OVR) performs veterinary toxicological diagnostic and analytical services for national public and private veterinary services, including those from neighboring countries. The prevalence of animal poisoning in South Africa and the region, whether intentional or accidental is unknown. In the current study, we surveyed archived diagnostic data generated in the laboratory over a five year period (2017 – 2022) using gas chromatography-mass spectrometry (GC-MS), to determine the presence and type of pesticides found in samples (n=194) associated with suspect poisoning cases. The samples analysed included baits, feed, water, and stomach contents of wildlife and domestic animals. Terbufos, a chemical compound used in insecticides and nematicides of the organophosphate family, was detected in 35% of the cases. It is highly toxic with an acute oral LD?? of 1.6 mg/kg and 5.5 mg/kg in male and female mice respectively. Of all the samples analysed, those from dogs were in the majority, which could have been as a result of intentional malicious poisoning or innocent ingestion of poisoned carcasses. Wide spread malicious poisoning of animals with Aldicarb, a carbamate pesticide and nemacide, contributed to it being withdrawn from South African markets in 2012. Our results show that Terbufos is currently the most frequent poison associated with dog casualties and fatalities, and warrant a review on accessibility and usage of products containing this chemical.