Main topic : Surveillance and control of emerging diseases
Immunity status of cattle after vaccination in Azerbaijan
ZEYNALOVA S. 1, ABDULLAYEVA R. 1, ABBASOV V. 1
1 3rd Biosafety Level Central Reference Laboratory, Ministry of Agriculture, Baku, Azerbaijan
Lumpy skin diseases (LSD) is a skin disease of farm animals. LSD, a DNA virus belonging to the Capripox genus in the Poxviridae family. The disease is characterized by fever, noduls on the skin, mucous membranes and internal organs. The severity of clinical signs depends on the strain of the virus and the breed of infected cattle (WOAH 2010).
For the first time, the disease was registered in Azerbaijan in 2014, and it spread rapidly among animals. Vaccination against the disease is currently carried out by the Agency of Agrarian Services. The purpose of the study is to determine the level of antibodies that have appeared in blood samples after vaccination. Determination of the amount of antibodies is an indicator of the effectiveness of vaccination, and based on these results, preventive measures are planned in advance.
Biosecurity and biodefense regulations. Collection, packaging and transportation of samples were carried out in accordance with biosafety rules (Chapter 1.1.4 Biosafety and biosecurity: Standard for managing biological risk in the veterinary laboratory and animal facilities).
Samples were collected from different regions by "random" sampling method. The regions of Bilasuvar, Ujar, Hajigabul, Imishli and Sabirabad were selected for the study. The blood of 10 vaccinated and 10 non-vaccinated cattle was collected from each region.
The result of the scientific research: the tension of immunity against nodular dermatitis was checked by serological-immunological (ELISA) examination. As a result, 51 vaccinated and 51 non-vaccinated bloods were tested.
New vaccination of animals (20-25 days ago) was taken into account in the calculations. Antibodies appear 21 days after vaccination.
According to the ELISA method, the results above 30% are positive, that is, they indicate the formation of antibodies.
Ujar region - it was in the range of 20-30%.
Bilasuvar region - titer was over 30% in 4 bloods.
Sabirabad region - titer in 1 blood was 19%.
Hajigabul region - titer in 1 blood was 24%.
Imishli region - titer was 13-36% in 7 bloods.
51 units of unvaccinated blood gave negative results. The amount of antibodies was below normal.
As a final result, the presence of low indicators in Hajigabul and Sabirabad region is associated with defects in the vaccination process. It is recommended to follow the "cold" chain principle.